Antigen

Antigen Class

TFs and others

Antigen

Myb

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

Lin- bone marrow cells

genotype

WT

cell type

Lin- bone marrow cells

chip antibody

Abcam ab76009

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells pellets were suspended in buffer (0.1% SDS, 1% Triton, 1 mM EDTA, 16.7 mM Tris pH 8.1, 167 mM NaCl) and were sonicated for 14 cycles (30s on/off) using Diagenode Bioruptor Pico. 1 ug of chromatin samples were used in each IP with 1.5 ug of antibody against Myb or rabbit normal IgG, and 20 ul of Protein A beads (NEB, S1425S). The washed beads were tagged with 2ul of TDE1 from Illumina (FC-121-1030) in a 30 ul reaction. The beads were reversed cross-linked overnight at 65 degree. The DNA was amplified for 12 cycles with Illumina primers. Libraries were sequenced single-end in an Illumina Nextseq 500

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

32411775

Reads aligned (%)

93.2

Duplicates removed (%)

31.1

Number of peaks

4584 (qval < 1E-05)

mm9

Number of total reads

32411775

Reads aligned (%)

93.0

Duplicates removed (%)

31.1

Number of peaks

4523 (qval < 1E-05)