Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Myb

Cell type

Cell type Class
Blood
Cell type
Bone Marrow Cells
MeSH Description
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Attributes by original data submitter

Sample

source_name
Lin- bone marrow cells
genotype
WT
cell type
Lin- bone marrow cells
chip antibody
Abcam ab76009

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells pellets were suspended in buffer (0.1% SDS, 1% Triton, 1 mM EDTA, 16.7 mM Tris pH 8.1, 167 mM NaCl) and were sonicated for 14 cycles (30s on/off) using Diagenode Bioruptor Pico. 1 ug of chromatin samples were used in each IP with 1.5 ug of antibody against Myb or rabbit normal IgG, and 20 ul of Protein A beads (NEB, S1425S). The washed beads were tagged with 2ul of TDE1 from Illumina (FC-121-1030) in a 30 ul reaction. The beads were reversed cross-linked overnight at 65 degree. The DNA was amplified for 12 cycles with Illumina primers. Libraries were sequenced single-end in an Illumina Nextseq 500

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
32411775
Reads aligned (%)
93.2
Duplicates removed (%)
31.1
Number of peaks
4584 (qval < 1E-05)

mm9

Number of total reads
32411775
Reads aligned (%)
93.0
Duplicates removed (%)
31.1
Number of peaks
4523 (qval < 1E-05)

Base call quality data from DBCLS SRA